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Western Blotting Reagents
Supplier:
GE Healthcare - Life Sciences
Description:
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Catalog Number:
(CA95040-068L)
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.
Catalog Number:
(89164-902)
Supplier:
Enzo Life Sciences
Description:
Whole-cell extract of human HL-60 leukemia cells.
Supplier:
GE Healthcare - Life Sciences
Description:
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Catalog Number:
(CA95038-568L)
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents
Supplier:
GE Healthcare - Life Sciences
Description:
CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.
Supplier:
GE Healthcare - Life Sciences
Description:
ECL Direct™ nucleic acid labelling and detection systems are based on the direct labelling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalysed breakdown of luminol. Suitable for fast and easy detection of medium to high target amounts in applications such as colony/plaque screens, dot blots and PCR product analyses.
Catalog Number:
(CA95038-570L)
Supplier:
GE Healthcare - Life Sciences
Description:
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
Supplier:
GE Healthcare - Life Sciences
Description:
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Supplier:
GE Healthcare - Life Sciences
Description:
AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Catalog Number:
(CAPI80196)
Supplier:
LIFE TECHNOLOGIES CORP CA
Description:
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Catalog Number:
(CAPI34012)
Supplier:
LIFE TECHNOLOGIES CORP CA
Description:
The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.
Catalog Number:
(CA95057-536)
Supplier:
G-Biosciences
Description:
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis
Supplier:
MilliporeSigma
Description:
A fluorogenic substrate for the detection of granzyme B and caspase-8 activity
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