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Cloning Kits and Ligation
Catalog Number:
(CA99902-306)
Supplier:
AGILENT TECHNOLOGIES (GENOMICS) CA
Description:
<i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
Supplier:
SGI - DNA Synthetic Genomics
Description:
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.
Supplier:
SGI - DNA Synthetic Genomics
Description:
Generate seamless plasmid DNA or BAC clones without restriction digestion.
Supplier:
SGI - DNA Synthetic Genomics
Description:
The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.
Supplier:
SGI - DNA Synthetic Genomics
Description:
BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.
Catalog Number:
(CA95040-312L)
Supplier:
GE Healthcare - Life Sciences
Description:
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
Supplier:
AGILENT TECHNOLOGIES (GENOMICS) CA
Description:
The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
Supplier:
AGILENT TECHNOLOGIES (GENOMICS) CA
Description:
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
Supplier:
SGI - DNA Synthetic Genomics
Description:
BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.
Supplier:
SGI - DNA Synthetic Genomics
Description:
The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.
Supplier:
SGI - DNA Synthetic Genomics
Description:
The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.
Supplier:
SGI - DNA Synthetic Genomics
Description:
Generate seamless plasmid DNA or BAC clones without restriction digestion.
Supplier:
SGI - DNA Synthetic Genomics
Description:
The Select DNA cloning Gibson assembly kits can generate Gibson clones starting with fragments and vector supplied by the end user.
Supplier:
Lucigen
Description:
The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.
Supplier:
AGILENT TECHNOLOGIES (GENOMICS) CA
Description:
The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.
Supplier:
AGILENT TECHNOLOGIES (GENOMICS) CA
Description:
For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.
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