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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-CA97065-200

NxGen™ T4 DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This e...

Product Image-CA101228-152

Taq DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotid...

Product Image-CA200064-420

9°N™ DNA Ligase, New England Biolabs

Catalog Number: (CA200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleoti...
Product Image-CA102715-942

ELECTROLIGASE®, New England Biolabs

Catalog Number: (CA102715-942)
Supplier: New England Biolabs (NEB)
Description: ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.
Product Image-CA101227-654

QUICK LIGATION™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature

Certificates

Product Image-CA76407-346

Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hyd...

Product Image-CA102500-174

T7 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

Product Image-CA102715-904

Thermostable 5' AppDNA/RNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

Product Image-CA102715-910

T4 RNA Ligase 2 Truncated, KQ, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Product Image-CA101417-942

QUICK BLUNTING™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligat...

Product Image-CA101640-956

T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Product Image-CA101228-182

T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

Certificates

Product Image-CA103218-906

NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs

Catalog Number: (CA103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly
Product Image-CA76299-644

NEB Cloning Competent E. coli Sampler, New England Biolabs

Catalog Number: (CA76299-644)
Supplier: New England Biolabs (NEB)
Description: The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.
Product Image-CA101417-962

T4 RNA Ligase 2 Truncated, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Product Image-CA102855-130

NEBNEXT® ULTRA™ Ligation Module, New England Biolabs

Catalog Number: (CA102855-130)
Supplier: New England Biolabs (NEB)
Description: The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.
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