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Blotting+Kits
Catalog Number:
(76235-516)
Supplier:
Rockland Immunochemical
Description:
AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
Catalog Number:
(CA95029-200)
Supplier:
G-Biosciences
Description:
G-Biosciences' HOOK™ Cell Surface Protein Isolation kit uses our HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting
Catalog Number:
(CA76102-544)
Supplier:
GE Healthcare - Life Sciences
Description:
The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.
Catalog Number:
(CA95057-536)
Supplier:
G-Biosciences
Description:
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis
Supplier:
GE Healthcare - Life Sciences
Description:
Hybond™ ECL™ gives excellent sensitivity, resolution, and low background for all labelling and detection systems (radioactive, non radioactive, and chromogenic), especially when used with ECL™, ECL™ Prime, and ECL™ Plex Western Blotting Detection kits and reagents. Special packaging features include interleaf on both sides of the membrane and air-tight resealable aluminium bag with label space for marking the 'opened on' date.
Supplier:
GE Healthcare - Life Sciences
Description:
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
Supplier:
G-Biosciences
Description:
Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis
Supplier:
G-Biosciences
Description:
G-Biosciences' femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining
Catalog Number:
(10764-990)
Supplier:
Prosci
Description:
The ACK2 monoclonal antibody specifically reacts with mouse CD117 (c-Kit receptor), a 145 kDa transmembrane tyrosine-kinase receptor encoded by the Kit gene. The c-Kit receptor, also known as stem cell factor receptor, is expressed on hematopoietic progenitor cells in adult bone marrow, in progenitors of erythroid and myeloid lineages, and precursors of B and T cells. CD117 enhances the proliferation and the differentiation of the hematopoietic progenitor cells and seems to enhance the development of T cells, as the c-Kit receptor and its ligand are expressed by the thymus.
Supplier:
IBI Scientific
Description:
Cost-effective phenol, guanidine isothiocyanate plus spin column system for purification of high-quality total RNA.
Applications: cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting
Catalog Number:
(10764-986)
Supplier:
Prosci
Description:
The ACK2 monoclonal antibody specifically reacts with mouse CD117 (c-Kit receptor), a 145 kDa transmembrane tyrosine-kinase receptor encoded by the Kit gene. The c-Kit receptor, also known as stem cell factor receptor, is expressed on hematopoietic progenitor cells in adult bone marrow, in progenitors of erythroid and myeloid lineages, and precursors of B and T cells. CD117 enhances the proliferation and the differentiation of the hematopoietic progenitor cells and seems to enhance the development of T cells, as the c-Kit receptor and its ligand are expressed by the thymus.
Catalog Number:
(10764-988)
Supplier:
Prosci
Description:
The ACK2 monoclonal antibody specifically reacts with mouse CD117 (c-Kit receptor), a 145 kDa transmembrane tyrosine-kinase receptor encoded by the Kit gene. The c-Kit receptor, also known as stem cell factor receptor, is expressed on hematopoietic progenitor cells in adult bone marrow, in progenitors of erythroid and myeloid lineages, and precursors of B and T cells. CD117 enhances the proliferation and the differentiation of the hematopoietic progenitor cells and seems to enhance the development of T cells, as the c-Kit receptor and its ligand are expressed by the thymus.
Supplier:
G-Biosciences
Description:
picoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase
Supplier:
Omega Bio-Tek
Description:
Isolated RNA can be used for Northern blotting, RT-PCR, nuclease protection assays, in vitro translation, and microarray analysis
Catalog Number:
(10813-666)
Supplier:
Prosci
Description:
ELISA: In a sandwich ELISA (assuming 100 uL/well), a concentration of 4.0-8.0 ug/mL of this antibody will detect at least 1000 pg/mL of recombinant human SCF when used with our biotinylated antigen affinity purified anti-human SCF (XP-5264Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/mL. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.
Catalog Number:
(89417-008)
Supplier:
Prosci
Description:
SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
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