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Isolation+and+Cleanup+HyClone+products+(Cytiva)
Catalog Number:
(CA95040-334L)
Supplier:
GE Healthcare - Life Sciences
Description:
Ready-To-Go™ You-Prime First-Strand beads are preformulated, single-dose reaction beads prepackaged in thin-walled 0,5 ml tubes compatible with most thermal cyclers. Ready-To-Go™ You-Prime First-Strand beads are used for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
Catalog Number:
(89359-538)
Supplier:
Genetex
Description:
Estrogen-related receptor alpha (ERR alpha), a NR3 Steroid Receptor, was isolated based on sequence similarity in its DNA-binding domain to estrogen receptor alpha (ER alpha). ERR alpha has been shown to regulate the promoters of lactoferrin, medium-chain acyl CoA dehydrogenase, osteopontin, and thyroid receptor alpha, and it may affect cellular energy balance and bone formation. ERR alpha binds as a monodimer to the extended half-site TNAAGGTCA and as a homodimer to the estrogen response element (ERE) and is a constitutive activator of the estrogen response element and the palindromic thyroid hormone response element (TRE(pal)) but not of the glucocorticoid response element (GRE). ERR alpha 1 is the major isoform expressed in human breast cancer cell lines. Recent studies have shown that Phe-329 is responsible for the constitutive activity of ERR alpha. ERR alpha is a potential biomarker for unfavorable clinical outcome and, possibly, hormonal insensitivity in breast tumors. ERR alpha status may be predictive of sensitivity to hormonal blockade therapy, and ERR alpha status may also be predictive of ErbB2-based therapy such as Herceptin. Moreover, ERR alpha may be a candidate target for therapeutic development. ERRalpha null mice have altered regulation of genes involved in adipogenesis.In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland, heart, intestine, bone, brown adipose tissue, heart, uterus, cervix, nerve, skeletal muscle, and vagina. ESTs have been isolated from human tissue libraries, including cancerous blood, brain, breast, cervix, colon, duodenum, eye, head/neck, kidney, liver, lung, ovary, pancreas, skeletal muscle, skin, stomach, and uterus, and normal adrenal, blood, brain, colon, embryo, eye, head/neck, heart, kidney, prostate, skeletal muscle, testis, and uterus. The ligands for ERR alpha are PPARgamma coactivator 1 (PGC-1) beta and flavone and isoflavone phytoestrogens.
Supplier:
VWR
Description:
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.
Catalog Number:
(CA28952359)
Supplier:
GE Healthcare - Life Sciences
Description:
GST SpinTrap™ columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST SpinTrap™ columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.
Catalog Number:
(CA97067-874)
Supplier:
GE Healthcare - Life Sciences
Description:
SP Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.
Supplier:
GE Healthcare - Life Sciences
Description:
SOURCE 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
Supplier:
GE Healthcare - Life Sciences
Description:
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
Catalog Number:
(CA95038-280L)
Supplier:
GE Healthcare - Life Sciences
Description:
ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Catalog Number:
(CAPIPA5-17989)
Supplier:
Thermo Scientific
Description:
This antibody is predicted to react with porcine and rat based on sequence homology. This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
Catalog Number:
(CAPISA19588)
Supplier:
Thermo Scientific
Description:
SA1-9588 contains 1 ml of antibody linked to agarose beads (50%) in PBS with 0. 02% sodium azide. This antibody reacts with the heavy and light chains of the immunoglobulin molecule. SA1-9588 immunogen is immunoglobulin isolated from chicken serum. To obtain optimal immunoprecipitation, incubate the beads with samples containing IgY antibodies-protein complex for at least 10 minutes. Binding capacity of the beads is approximately 450 µg to 500 µg IgY per ml solid beads. Thermo Scientific Pierce Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e. g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i. e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc. ) to generate highly specific reagents.
Supplier:
GE Healthcare - Life Sciences
Description:
Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).
Supplier:
GE Healthcare - Life Sciences
Description:
HiTrap Butyl HP are prepacked Butyl Sepharose High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elution conditions.
Catalog Number:
(CA95020-700)
Supplier:
HiMedia
Description:
For the cultivation of acid tolerant microorganisms from canned foods.
Catalog Number:
(CA95056-288L)
Supplier:
GE Healthcare - Life Sciences
Description:
His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.
Catalog Number:
(CA90000-182)
Supplier:
BD
Description:
Mannitol Salt Agar is a selective and differential medium for the detection and enumeration of staphylococci from clinical and nonclinical specimens.
Catalog Number:
(CA90003-102)
Supplier:
BD
Description:
Azide Dextrose Broth is used for cultivating streptococci in water and wastewater.
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