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chromatography+columns+HyClone+products+(Cytiva)
Supplier:
GE Healthcare - Life Sciences
Description:
IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
Supplier:
GE Healthcare - Life Sciences
Description:
Sera-Mag SpeedBeads Protein A/G Magnetic Particles enable magnetic isolation of IgA and IgG proteins in a single isolation. Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for manual and automated magnetic isolation of affinity bound antibodies or antigens to Protein A/G. These particles can be used for isolating antibodies from serum, cell culture supernatants, or ascites, and for immunoprecipitation and co-immunoprecipitation of antigens from cell or tissue extracts.
Supplier:
GE Healthcare - Whatman
Description:
AcroCap™ devices are presterilized, disposable filter units ideal for sterilization or clarification of up to 3 L of tissue culture media or other critical fluids.
Supplier:
GE Healthcare - Whatman
Description:
Ideal for prefiltration of difficult-to-filter solutions and serum.
Catalog Number:
(CA95017-782L)
Supplier:
GE Healthcare - Life Sciences
Description:
Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.
Supplier:
GE Healthcare - Life Sciences
Description:
Immobiline™ DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application. IPG strips contain a preformed pH gradient immobilised in precast polyacrylamide gels on a plastic backing. A comprehensive range of overlapping IPG strips covering narrow, medium, and wide pH ranges are available in various strip lengths, and the strips are individually packaged and bar coded for convenient handling and sample tracking.
Catalog Number:
(CA95038-574L)
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.
Catalog Number:
(CA76102-544)
Supplier:
GE Healthcare - Life Sciences
Description:
The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.
Supplier:
GE Healthcare - Whatman
Description:
Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pre treatment steps are necessary.
Catalog Number:
(CA97067-754)
Supplier:
GE Healthcare - Life Sciences
Description:
CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.
Catalog Number:
(CA11010-722)
Supplier:
GE Healthcare - Life Sciences
Description:
His MultiTrap™ TALON® multiwell plates are designed for high-throughput screening of histidine-tagged proteins from clarified cell lysates. His MultiTrap™ TALON® prepacked plates are manufactured with high reproducibility with regards to the well and media volumes in order to enable consistent results. The average purity of His MultiTrap TALON® is >85% with yields up to 1 mg/well. Leakage of cobalt ions from TALON® Superflow™ medium is negligible. His MultiTrap™ TALON® is used manually with vacuum or centrifugation, or in a liquid handling station.
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham Hybond P 0.2 PVDF is a 0.2 μm pore size hydrophobic membrane with high physical strength. This results in significant handling advantages over unsupported nitrocellulose and makes the membrane highly suitable for stripping and reprobing. The small pore size of the membrane minimizes ‘blow-through’ and increases protein binding over a wide range of molecular weights. Ideal for use with standard colorimetric and chemiluminescent detection methods for proteins of <Mr 20000.
Catalog Number:
(CA28143-978)
Supplier:
GE Healthcare - Whatman
Description:
These syringe filters are ideal for filtration of gas and organic solvents.
Supplier:
GE Healthcare - Life Sciences
Description:
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
Supplier:
GE Healthcare - Life Sciences
Description:
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
Catalog Number:
(CA95017-030)
Supplier:
GE Healthcare - Life Sciences
Description:
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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