Plasmid DNA ManufacturingEstablishing a reliable and cost-effective source of high quality pDNA, both Critical Reagent Grade or GMP grade, is of critical importance to cell & gene therapy (CGT) companies, and companies producing mRNA vaccines and therapies. Within the CGT field a robust pipeline and increasing capital investment are driving the need for increased plasmid production to support growing CGT and mRNA therapeutics market. Avantor provides CGT and mRNA biopharma companies with large volumes of high-quality Critical Reagent Grade or GMP plasmid DNA. Produced in a process optimized for high yield of high quality plasmid DNA, this service enables scale up of plasmid DNA and supports speed to market. This plasmid DNA manufacturing service provides the following:
Find out more about our pDNA production service for viral vector and mRNA manufacturersWhat is plasmid DNA (pDNA)?A plasmid is a small, circular double-stranded DNA molecule found in bacteria and other microbes, where it is separate from chromosomal DNA. They typically contain a small number of genes and in nature, they can carry genes that confer advantage to a microbe such as genes for antibiotic resistance. How is pDNA used in viral vector bioprocessing?In the viral vector production workflow for gene therapies or for genetic modification of cell therapies, plasmids are used to transfect a cell line with the genetic information required to produce a viral vector containing the therapeutic gene of choice. Generally three or four plasmids are used, including structural or ‘helper’ plasmids and a plasmid containing the therapeutic gene of interest. How is pDNA used in mRNA Vaccine & Therapy manufacturing?In the mRNA vaccine & therapies workflow plasmid DNA is the template for mRNA production via the linearization and enzymatic in vitro transcription (IVT) reaction. mRNA produced in this can be used to induce the production of a viral protein that then triggers an immune response, in the case of mRNA vaccines, or it can be used to induce cellular production of a protein to replace one that is defective or missing. What is the difference between Critical Reagent Grade and cGMP grade plasmid DNA?Critical Reagent Grade and cGMP pDNA are the two grades of plasmid DNA available through this plasmid DNA manufacturing service. They are both produced using the same starting materials, process, production space and specification. |