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jetMESSENGER™ is a mRNA transfection reagent that has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard to transfect, allowing relevant gene expression experiments in all cell types.
Unmatched transfection efficiency
High efficiency on a wide variety of difficult to transfect cells
Outperform DNA transfection by switching to mRNA
Extremely gentle on cells
No risk of genome integration
Perfectly suited for CRISPR/Cas9 gene editing, iPS generation, stem cell differentiation and immunotherapy assays
jetMESSENGER™ has been designed to transfect cells that are slow dividing or that have developed specific mechanisms such as primary cells, neurons, suspension cells and various cancer cell lines. jetMESSENGER™ is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
no risk of genome integration, hence no genome modification of the transfected cell
no promoter regulation issue
no need to reach the nucleus for efficient expression
more gentle process
jetMESSENGER™ has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard to transfect, finally allowing relevant gene expression experiments in almost all cell types
jetMESSENGER™ outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of eGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions were used according to the manufacturers’ recommendation.
With jetMESSENGER™, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER™ is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells.
jetMESSENGER™ leads to high transfection efficiency in a wide variety of cell types.
By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER™ allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER™ operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained. Make your transfection experiments physiologically relevant!
jetMESSENGER™ provides a better cell viability and a higher protein expression than DNA transfection. Primary Rat Cortical neurons, Hep G2 and mouse stem cells were analyzed 48 h after transfection using phase contrast and fluorescent microscopy. The transfections were performed with jetMESSENGER™/eGFP mRNA (5meC, pseudo-uridine, Trilink™) and L2K/eGFP plasmid DNA according to the manufacturers’ recommendations for each reagent.
Examples of citations
Viegas C.S.B., Costa R.M., Santos L., Videira P.A., Silva Z., Araújo N., Macedo A.L., Matos AP., Vermeer C., Simes D.C. (2017). Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases. PLoS Ones, DOI:10.1371/journal.pone.0177829
Sultana N., Magadum A., Hadas Y., Kondrat J., Singh N., Youssef E., Calderon D., Chepurko E., Dubois N., Hajjar RJ., Zangi L. (2017). Optimizing Cardiac Delivery of Modified mRNA. Mol Ther, DOI:10.1016/j.ymthe.2017.03.016
Testimonials
"Application of jetMESSENGER reagent to introduce modified RNA molecules into primary lung mouse endothelial cells (a cell population which is generally hard to transfect with DNA/RNA using non-viral methodology) resulted with the highest transfection rate (>70%) and with the lowest toxicity effects as compared to other tested RNA/DNA transfection reagents (jetPEI, TransIT, & RNAiMAX)." Itkin T., Weill Cornell Medicine, New York, United States
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